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瑞士改进轧制技术肠组织准备免疫组化和免疫分析
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Biology
Improved Swiss-rolling Technique for Intestinal Tissue Preparation for Immunohistochemical and Immunofluorescent Analyses
Please note that all translations are automatically generated.
Click here for the English version.
瑞士改进轧制技术肠组织准备免疫组化和免疫分析
DOI:
10.3791/54161-v
•
07:42 min
•
July 13, 2016
•
Agnieszka B. Bialkowska*
1
,
Amr M. Ghaleb*
1
,
Mandayam O. Nandan
,
Vincent W. Yang
2
1
Department of Medicine
,
Stony Brook University School of Medicine
,
2
Department of Physiology & Biophysics
,
Stony Brook University School of Medicine
Chapters
00:05
Title
00:48
Colon and Small Intestine Preparation
04:38
Swiss Rolling
05:55
Results: Stained Swiss Roll Intestinal Preparations
07:09
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
准确识别，并沿着肠道粘膜内层的上皮细胞的位置是必要的，以定义不同的细胞谱系。肠组织的正常成像识别的蛋白表达模式与最大分辨率是至关重要的。本研究旨在描绘处理小鼠肠组织的最佳方法和条件。
Tags
Immunohistochemical
Immunofluorescent
Swiss Rolling Technique
Fixation Method
Mouse Intestine
Abdominal Muscles
Colon
Small Intestine
Cecum
Modified Bouin’s Fixative
Gavage Needle
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