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Immunology and Infection
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在剪切粘结力的测定T淋巴细胞粘附分子相互作用的研究
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Immunology and Infection
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JoVE Journal
Immunology and Infection
Assay of Adhesion Under Shear Stress for the Study of T Lymphocyte-Adhesion Molecule Interactions
Please note that all translations are automatically generated.
Click here for the English version.
在剪切粘结力的测定T淋巴细胞粘附分子相互作用的研究
DOI:
10.3791/54203-v
•
07:40 min
•
June 29, 2016
•
Marianne Strazza
,
Inbar Azoulay-Alfaguter
,
Michael Peled
,
Adam Mor
2
1
Department of Medicine
,
New York University School of Medicine
,
2
Department of Pathology
,
New York University School of Medicine
Chapters
00:05
Title
00:48
Chinese Hamster Ovary (CHO)-Intercellular Adhesion Molecule (ICAM) Cell Plating
01:50
T Cell Preparation
02:46
Input/Output Hosing and Syringe Pump Preparation
03:59
Flow Chamber Loading and Cell Stimulation
05:53
Results: SDF-1alpha Induced T Cell Adhesion to ICAM-1 Under Shear Stress
06:51
Conclusion
Summary
Automatic Translation
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Automatic Translation
这种流动粘附实验提供了T细胞的上皮细胞间的相互作用一个简单的,高冲击的模型。的注射器泵用于产生剪切应力,和共聚焦显微镜捕捉图像进行定量。这些研究的目标是使用流动条件,有效地量化的T细胞粘附。
Tags
Adhesion
Shear Stress
T Lymphocyte
Adhesion Molecule
Integrin
LFA1
CHO-ICAM
T Cells
CFSE
Flow Chamber
Live Imaging
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