Observation and Quantification of Telomere and Repetitive Sequences Using Fluorescence In Situ Hybridization (FISH) with PNA Probes in Caenorhabditis elegans

Beomseok Seo; Junho Lee

doi:10.3791/54224

/

Observation et la quantification des télomères et des séquences répétitives utilisant Fluorescence In Situ Hybridation (FISH) avec PNA Sondes en Caenorhabditis elegans</em

Observation and Quantification of Telomere and Repetitive Sequences Using Fluorescence <em>In Situ</em> Hybridization (FISH) with PNA Probes in <em>Caenorhabditis elegans</em>

JoVE Journal
Biology

A subscription to JoVE is required to view this content. Sign in or start your free trial.

JoVE Journal Biology

Observation and Quantification of Telomere and Repetitive Sequences Using Fluorescence In Situ Hybridization (FISH) with PNA Probes in Caenorhabditis elegans

Observation et la quantification des télomères et des séquences répétitives utilisant Fluorescence In Situ Hybridation (FISH) avec PNA Sondes en Caenorhabditis elegans</em

Please note that all translations are automatically generated. Click here for the English version.

10,254 Views

•

10:01 min

•

August 04, 2016

DOI:

10.3791/54224-v

DOI:

10.3791/54224-v

•

10:01 min

August 04, 2016

•

25 Views

Beomseok Seo¹, Junho Lee^1,2,3

¹Institute of Molecular Biology and Genetics (IMBG),Seoul National University, ²Department of Biological Sciences,Seoul National University, ³Department of Biophysics and Chemical Biology,Seoul National University

Transcript

Summary

We report a concise procedure of fluorescence in situ hybridization (FISH) in the gonad and embryos of Caenorhabditis elegans for observing and quantifying repetitive sequences. We successfully observed and quantified two different repetitive sequences, telomere repeats and template of alternative lengthening of telomeres (TALT).