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鲁棒戊糖发酵酵母的木质纤维素生物转化的演进技术乙醇
JoVE Journal
Bioengineering
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JoVE Journal Bioengineering
Techniques for the Evolution of Robust Pentose-fermenting Yeast for Bioconversion of Lignocellulose to Ethanol
DOI:

14:53 min

October 24, 2016

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Chapters

  • 00:05Title
  • 01:40Enrichment of Robust Derivatives during Serial Transfer on AFEX CSH
  • 03:42Isolation of Single Cell Tolerant Derivatives after Enrichment on AFEX CSH
  • 04:58Performance Evaluation of AFEX CSH Tolerant Derivatives Compared to Parent
  • 07:15Applying Continuous Culture to Select for Ethanol-challenged Xylose Utilization
  • 09:43Isolation of Single-cell Colonies That Utilize Xylose in PSGHL When Ethanol Is Present
  • 11:20Results: Derivatives of S. stipitis Parent Strain Have Improved Efficiency in Converting Hydrolyzates to Ethanol
  • 12:54Conclusion

Summary

Automatic Translation

适应进化和隔离技术进行了描述和展示,得到了能够迅速消耗己糖和酶戊混合糖的糖化undetoxified水解和超过40克/升乙醇积聚Scheffersomyces树干毕赤酵母株的衍生物NRRL Y-7124。

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