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에탄올에 리그 노 셀룰로즈의 생물에 대한 강력한 펜 토스 발효 효모의 진화를위한 기술
JoVE Journal
Bioengineering
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JoVE Journal Bioengineering
Techniques for the Evolution of Robust Pentose-fermenting Yeast for Bioconversion of Lignocellulose to Ethanol
DOI:

14:53 min

October 24, 2016

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Chapters

  • 00:05Title
  • 01:40Enrichment of Robust Derivatives during Serial Transfer on AFEX CSH
  • 03:42Isolation of Single Cell Tolerant Derivatives after Enrichment on AFEX CSH
  • 04:58Performance Evaluation of AFEX CSH Tolerant Derivatives Compared to Parent
  • 07:15Applying Continuous Culture to Select for Ethanol-challenged Xylose Utilization
  • 09:43Isolation of Single-cell Colonies That Utilize Xylose in PSGHL When Ethanol Is Present
  • 11:20Results: Derivatives of S. stipitis Parent Strain Have Improved Efficiency in Converting Hydrolyzates to Ethanol
  • 12:54Conclusion

Summary

Automatic Translation

적응 진화와 분리 기술을 설명하고 신속하게 육탄 당과 효소의 오탄당의 혼합 당이 undetoxified 가수 분해물을 당화 및 40g / L의 에탄올을 통해 축적 소비 할 수있는 Scheffersomyces stipitis 변형의 파생 상품 NRRL Y-7124을 얻었다 증명된다.

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