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Cancer Research
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如何学习基底膜刚度生物物理触发在前列腺癌和其他与年龄相关的病理或代谢性疾病
JoVE Journal
Cancer Research
This content is Free Access.
JoVE Journal
Cancer Research
How to Study Basement Membrane Stiffness as a Biophysical Trigger in Prostate Cancer and Other Age-related Pathologies or Metabolic Diseases
Please note that all translations are automatically generated.
Click here for the English version.
如何学习基底膜刚度生物物理触发在前列腺癌和其他与年龄相关的病理或代谢性疾病
DOI:
10.3791/54230-v
•
13:18 min
•
September 20, 2016
•
Mercedes Rodriguez-Teja
,
Claudia Breit
,
Mitchell Clarke
,
Kamil Talar
,
Kai Wang
,
Mohammad A. Mohammad
,
Sage Pickwell
,
Guillermina Etchandy
,
Graeme J. Stasiuk
,
Justin Sturge
1
Departamento de Genética, Facultad de Medicina
,
Universidad de la República (UDELAR)
,
2
Department of Mechanistic Cell Biology
,
Max Planck Institute of Molecular Physiology
,
3
School of Biological, Biomedical & Environmental Sciences
,
University of Hull
Chapters
00:05
Title
01:38
Induction of Basement Membrane Stiffness Induced by GLA Treatment
04:18
Photometric and SDS-PAGE Analysis of Reconstituted Basement Membrane
06:27
Immunofluorescent Microscopy Analysis
09:09
3D Culture of Prostate Gland Acini and Prostate Tumor Cell Aggregates on Native and Stiff Reconstituted Basement Membrane
10:41
Results: Prostate Cell Reactions to Changes in Basement Membrane Stiffness
12:02
Conclusion
Summary
Automatic Translation
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Automatic Translation
在这里,我们解释模型生物物理微环境,其中交联和提高晚期糖基化产物(AGEs)引起的基底膜(BM)的刚度病理意义的协议。
Tags
Basement Membrane
Stiffness
Thickness
Advanced Glycation
Prostate Cancer
Metastasis
Age-related Pathologies
Metabolic Diseases
Cell Invasion
3D Cell Culture
Cross-linking
Biophysical Trigger
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