Residue-specific Incorporation of Noncanonical Amino Acids into Model Proteins Using an Escherichia coli Cell-free Transcription-translation System

Emanuel G. Worst; Matthias P. Exner; Alessandro De Simone; Marc Schenkelberger; Vincent Noireaux; Nediljko Budisa; Albrecht Ott

doi:10.3791/54273

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La incorporación de residuos específicos de no canónicos aminoácidos en las proteínas modelo usando una Escherichia coli Sistema de transcripción-traducción libre de células

Residue-specific Incorporation of Noncanonical Amino Acids into Model Proteins Using an <em>Escherichia coli</em> Cell-free Transcription-translation System

JoVE Journal
Biology

This content is Free Access.

JoVE Journal Biology

Residue-specific Incorporation of Noncanonical Amino Acids into Model Proteins Using an Escherichia coli Cell-free Transcription-translation System

La incorporación de residuos específicos de no canónicos aminoácidos en las proteínas modelo usando una Escherichia coli Sistema de transcripción-traducción libre de células

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15,945 Views

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11:47 min

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August 01, 2016

DOI:

10.3791/54273-v

DOI:

10.3791/54273-v

•

11:47 min

August 01, 2016

•

15916 Views

Emanuel G. Worst¹, Matthias P. Exner², Alessandro De Simone², Marc Schenkelberger¹, Vincent Noireaux³, Nediljko Budisa², Albrecht Ott¹

¹Department of Experimental Physics,Saarland University ²Institute of Chemistry,Technische Universität Berlin ³School of Physics and Astronomy,University of Minnesota

Summary

An easy-to-use, cell-free expression protocol for the residue-specific incorporation of noncanonical amino acid analogs into proteins, including downstream analysis, is presented for medical, pharmaceutic, structural and functional studies.