Rest-specifikke Inkorporering af Noncanonical Aminosyrer i Model Proteiner Brug en<em> Escherichia coli</em> Cellefri transskription-translationssystem
An easy-to-use, cell-free expression protocol for the residue-specific incorporation of noncanonical amino acid analogs into proteins, including downstream analysis, is presented for medical, pharmaceutic, structural and functional studies.