Rest-spesifikk innlemmelse av Noncanonical Amino Acids inn Modell Proteiner Bruke en<em> Escherichia coli</em> Cell-free transkripsjon-oversettelse System
An easy-to-use, cell-free expression protocol for the residue-specific incorporation of noncanonical amino acid analogs into proteins, including downstream analysis, is presented for medical, pharmaceutic, structural and functional studies.