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工程三维上皮组织细胞外基质中嵌入
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Bioengineering
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JoVE Journal Bioengineering
Engineering Three-dimensional Epithelial Tissues Embedded within Extracellular Matrix
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工程三维上皮组织细胞外基质中嵌入

DOI:
10.3791/54283-v

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08:49 min

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July 10, 2016

•

,
1Chemical and Biological Engineering,Princeton University, 2Molecular Biology,Princeton University

Chapters

  • 00:05Title
  • 00:49Preparation of Elastomeric Stamps for 3D Micropatterning
  • 02:48Preparation of 3D Epithelial Tissues
  • 06:58Results: 3D Culture System Provides Insight to Growth Factor Mediated Branching Morphogenesis
  • 07:58Conclusion

Summary

Automatic Translation

Automatic Translation

该原稿描述了软基于光刻技术工程师三维（3D）的均匀阵列由细胞外基质包围限定的几何形状的上皮组织。这种方法是适合于多种细胞类型和实验的上下文，并允许对于相同重复的高通量筛选。

Tags

Extracellular MatrixBranching MorphogenesisCollective Cell BehaviorPhotolithographyPDMSMoldStamp

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