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Biology
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核酸的分离过滤:一种简单快速的DNA提取方法
JoVE Journal
Biology
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JoVE Journal
Biology
Filtration Isolation of Nucleic Acids: A Simple and Rapid DNA Extraction Method
Please note that all translations are automatically generated.
Click here for the English version.
核酸的分离过滤:一种简单快速的DNA提取方法
DOI:
10.3791/54289-v
•
07:56 min
•
August 06, 2016
•
Sally M. McFall
,
Mário F. Neto
,
Jennifer L. Reed
,
Robin L. Wagner
1
Center for Innovation in Global Health Technologies (CIGHT), Department of Biomedical Engineering
,
Northwestern University
,
2
Pritzker School of Medicine
,
The University of Chicago
Chapters
00:05
Title
01:08
Preparation of FINA Sample Preparation Module
02:27
Preparation of qPCR Tube
03:12
Preparation of Contrived Specimen
04:00
FINA Extraction
05:57
Results: FINA Enables Efficient Detection of Proviral HIV DNA from Whole Blood
07:20
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
在这里,我们描述了一个简单,快速的基于纸张的DNA提取的HIV前病毒DNA的全血通过定量PCR检测方法。这种协议可以在检测其他遗传标记或使用替代的扩增方法被扩展为使用。
Tags
Filtration Isolation
Nucleic Acid Extraction
DNA Extraction
Rapid DNA Extraction
Paper-based DNA Extraction
FINA Method
PCR Amplification
HIV Proviral DNA
Quantitative PCR
Sample Preparation Module
Blotting Pad
Paraffin Film
Glass Blood Separator Membrane
Double-coated Polyester Diagnostic Tape
PCR Tube
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