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1Molecular Imaging and Neurovascular Research Laboratory,Dongguk University College of Medicine, 2Biomedical Research Center,Korea Institute of Science and Technology, 3Research Institute of Advanced Materials, Department of Materials Science and Engineering,Seoul National University, 4Departments of Radiology and Cancer Systems Imaging,University of Texas M.D. Anderson Cancer Center
Chapters
- 00:05Title
- 01:20Exogenously Formed Clot Preparation and Fluorescent Marker Labeling
- 02:55Thromboembolic Stroke Model Preparation
- 05:42In Vivo MicroCT Cerebral Thrombus Imaging
- 06:36Ex Vivo Near Infrared Fluorescent (NIRF) Thrombus Imaging and MicroCT Thrombus Area Imaging Quantification
- 09:48Results: Representative Direct Cerebral Thrombus Imaging before and after Thrombolysis Activation
- 11:28Conclusion
इस प्रोटोकॉल मस्तिष्क thromboemboli visualizing के लिए संयुक्त लगभग अवरक्त फ्लोरोसेंट (NIRF) इमेजिंग और सूक्ष्म गणना टोमोग्राफी (microCT) के आवेदन का वर्णन है। इस तकनीक thrombus बोझ और विकास की मात्रा का ठहराव की अनुमति देता है। NIRF इमेजिंग तकनीक fluorescently, excised मस्तिष्क में थक्का लेबल visualizes जबकि microCT तकनीक सोने-नैनोकणों का उपयोग कर रहने वाले जानवरों के अंदर thrombus visualizes।
