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Chapters
- 00:05Title
- 00:50Culture of Clonal Sample Neurospheres
- 02:07Preparation of Neurosphere-conditioned Medium
- 04:20Transfer of Sample Neurospheres to the 50-well-chambered Coverslip
- 06:25Differentiation of Sample Neurospheres
- 07:18Fixing the Differentiated Neurospheres
- 08:16Results: Differentiation of Clonal Neurospheres and Enumeration of NSC Frequency
- 09:28Conclusion
Neurale stamceller (NSC) refererer til celler, som kan forny sig selv og differentiere til de tre neurale slægter. Her beskriver vi en protokol til at bestemme NSC frekvens i en given celle population ved hjælp neurosfære dannelse og differentiering under klonede betingelser.
