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使用克隆测定神经干细胞的计数。
JoVE Journal
Neuroscience
This content is Free Access.
JoVE Journal
Neuroscience
Enumeration of Neural Stem Cells Using Clonal Assays
Please note that all translations are automatically generated.
Click here for the English version.
使用克隆测定神经干细胞的计数。
DOI:
10.3791/54456-v
•
10:32 min
•
October 04, 2016
•
Gunaseelan Narayanan
,
Yuan Hong Yu
,
Muly Tham
,
Hui Theng Gan
,
Srinivas Ramasamy
,
Shvetha Sankaran
,
Srivats Hariharan
,
Sohail Ahmed
1
Neural Stem Cell Laboratory, Institute of Medical Biology
,
Agency for Science, Technology and Research (A*STAR)
Chapters
00:05
Title
00:50
Culture of Clonal Sample Neurospheres
02:07
Preparation of Neurosphere-conditioned Medium
04:20
Transfer of Sample Neurospheres to the 50-well-chambered Coverslip
06:25
Differentiation of Sample Neurospheres
07:18
Fixing the Differentiated Neurospheres
08:16
Results: Differentiation of Clonal Neurospheres and Enumeration of NSC Frequency
09:28
Conclusion
Summary
Automatic Translation
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Automatic Translation
神经干细胞(NSCs)是指细胞,其能够自我更新和分化为三个神经谱系。在这里,我们描述了一个协议,以确定在使用克隆的条件下神经球的形成和分化的给定的细胞群体NSC频率。
Tags
Neural Stem Cells
Clonal Assays
Neurosphere Formation
Neural Stem Cell Markers
Neural Progenitors
E14.5 Mouse
NSC Growth Medium
Trypan-blue Staining
Hemocytometer
Poly-L-lysine
Laminin
NSC Differentiation Medium
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