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Análise em profundidade Physiological de populações de células definido no aguda Tissue Fatias do mouse Vomeronasal Organ
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
In-depth Physiological Analysis of Defined Cell Populations in Acute Tissue Slices of the Mouse Vomeronasal Organ
DOI:

10:11 min

September 10, 2016

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Chapters

  • 00:05Title
  • 00:42VNO Dissection and Embedding
  • 04:50Coronal VNO Tissue Slicing
  • 05:48Single-cell Electrophysiological Recordings
  • 08:03Results: Isolated Voltage-gated Na+ and Ca2+ Currents as well as Spike Discharge
  • 09:24Conclusion

Summary

Automatic Translation

Here, we describe a physiological approach that allows identification and in-depth analysis of a defined population of sensory neurons in acute coronal tissue slices of the mouse vomeronasal organ using whole-cell patch-clamp recordings.

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