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Chapters
- 00:05Title
- 00:33Sputum Processing
- 01:31Chambered Coverglass Method for Biofilm Formation
- 02:46Staining Biofilms and Confocal Microscopy
- 03:46Results: Clear Effects of Sputum on Biofilm
- 04:38Conclusion
This protocol describes the visualization of biofilm development following exposure to host-factors using a slide chamber model. This model allows for direct visualization of biofilm development as well as analysis of biofilm parameters using computer software programs.
