9,731 Views
•
10:51 min
•
December 05, 2016
DOI:
Transcript
Automatically generated
Summary
Automatically generated
Temperature-sensitive (ts) lethal mutants are valuable tools to identify and analyze essential functions. Here we describe methods to generate and classify ts lethal mutants in high throughput.
Related Videos
10:12
A Robotic Platform for High-throughput Protoplast Isolation and Transformation
Related Videos
14160 Views
10:37
Perturbations of Circulating miRNAs in Irritable Bowel Syndrome Detected Using a Multiplexed High-throughput Gene Expression Platform
Related Videos
8204 Views
08:25
Using a Fluorescent PCR-capillary Gel Electrophoresis Technique to Genotype CRISPR/Cas9-mediated Knockout Mutants in a High-throughput Format
Related Videos
13667 Views
08:28
High Fat Diet Feeding and High Throughput Triacylglyceride Assay in Drosophila Melanogaster
Related Videos
11108 Views
10:05
Generation of Native Chromatin Immunoprecipitation Sequencing Libraries for Nucleosome Density Analysis
Related Videos
22002 Views
13:03
Robust DNA Isolation and High-throughput Sequencing Library Construction for Herbarium Specimens
Related Videos
10463 Views
07:51
High-throughput Identification of Synergistic Drug Combinations by the Overlap2 Method
Related Videos
11591 Views
08:08
Formaldehyde-assisted Isolation of Regulatory Elements to Measure Chromatin Accessibility in Mammalian Cells
Related Videos
11204 Views
10:12
High-throughput DNA Extraction and Genotyping of 3dpf Zebrafish Larvae by Fin Clipping
Related Videos
13849 Views
07:11
Digital PCR-based Competitive Index for High-throughput Analysis of Fitness in Salmonella
Related Videos
9519 Views
Read Article
Cite this Article
Breker, M., Lieberman, K., Tulin, F., Cross, F. R. High-Throughput Robotically Assisted Isolation of Temperature-sensitive Lethal Mutants in Chlamydomonas reinhardtii. J. Vis. Exp. (118), e54831, doi:10.3791/54831 (2016).
Copy