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باستخدام سقالة الليبوزومات لإعادة تفاعلات البروتين البروتين، الدهون القريب<em> في المختبر</em
JoVE Journal
Biochemistry
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JoVE Journal Biochemistry
Using Scaffold Liposomes to Reconstitute Lipid-proximal Protein-protein Interactions In Vitro
DOI:

08:53 min

January 11, 2017

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Chapters

  • 00:05Title
  • 00:58Scaffold Liposome Preparation
  • 02:49Use of Scaffold Liposomes for Protein Binding Analysis
  • 04:25Use of Scaffold Liposomes for Enzymatic Assay
  • 06:08Results: Reconstitution of Lipid-proximal Protein-protein Interactions via Scaffold Liposomes
  • 08:06Conclusion

Summary

Automatic Translation

This paper describes a method for assessing the interactions and assemblies of integral membrane proteins in vitro with various partner factors in a lipid-proximal environment.

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