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Cell Lineage Analyses and Gene Function Studies Using Twin-spot MARCM
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Chapters
- 00:05Title
- 00:53Breeding tsMARCM-ready Flies to Generate tsMARCM Clones
- 02:25Preparation, Staining, and Mounting of Fly Brains Containing tsMARCM Clones
- 04:35Results: Neural Lineage Analysis Using Four tsMARCM Clones
- 05:55Conclusion
Here, we present a protocol for a mosaic labeling technique that permits the visualization of neurons derived from a common progenitor cell in two distinct colors. This facilitates neural lineage analysis with the capability of birth-dating individual neurons and studying gene function in the same neurons of different individuals.
