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Journal
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Developmental Biology
/
在
嵌合斑马鱼胚胎的个别视网膜祖细胞转基因表达的
体内
成像来研究细胞非自治的影响
JoVE Journal
Developmental Biology
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JoVE Journal
Developmental Biology
In Vivo
Imaging of Transgenic Gene Expression in Individual Retinal Progenitors in Chimeric Zebrafish Embryos to Study Cell Nonautonomous Influences
Please note that all translations are automatically generated.
Click here for the English version.
在
嵌合斑马鱼胚胎的个别视网膜祖细胞转基因表达的
体内
成像来研究细胞非自治的影响
DOI:
10.3791/55490-v
•
10:36 min
•
March 22, 2017
•
Stefanie Dudczig
2
,
Peter D. Currie
,
Lucia Poggi
,
Patricia R. Jusuf
2
1
School of Biosciences
,
The University of Melbourne
,
2
Australian Regenerative Medicine Institute (ARMI)
,
Monash University
,
3
The David J Apple Center for Vision Research, Department of Ophthalmology
,
Heidelberg University
Chapters
00:05
Title
00:52
Embryo Microinjection
02:53
Preparation for Transplantation
04:59
Chimera Generation via Blastula Transplantation
06:37
Mounting for Live Imaging
08:27
Results: Transgene Expression in Retinal Progenitors from Donor Cells
09:34
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
在不同的遗传背景的个体WT视网膜祖细胞的现场跟踪允许细胞非自主性的信令神经过程中贡献进行评估。这里,通过胚胎移植
和体内
的时间推移共焦成像的基因敲除,嵌合体的产生的组合物用于此目的。
Tags
In Vivo Imaging
Transgenic Gene Expression
Retinal Progenitor Cells
Chimeric Zebrafish Embryos
Cell Non-autonomous Influences
Fate Specification
Microinjection
H2AGFP
H2BRFP
MRNA
Fluorescence Microscopy
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