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体外成像和荧光标记的GnRH类似物的药物靶向性的定量分析
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Cancer Research
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JoVE Journal Cancer Research
In Vitro Imaging and Quantification of the Drug Targeting Efficiency of Fluorescently Labeled GnRH Analogues
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体外成像和荧光标记的GnRH类似物的药物靶向性的定量分析

DOI:
10.3791/55529-v

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10:36 min

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March 21, 2017

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, , , , ,
1MTA-SE Pathobiochemistry Research Group,Semmelweis University, 2Department of Medical Chemistry, Molecular Biology and Pathobiochemistry,Semmelweis University, 3MTA-ELTE Research Group of Peptide Chemistry,Eötvös Loránd University

Chapters

  • 00:05Title
  • 01:07GnRH Uptake
  • 02:39Type I GnRH Receptor Expression
  • 05:39Fluorescence Microscopy Imaging
  • 06:26FACS-assisted GnRH Quantification
  • 08:00Results: GnRH I Uptake and Type I GnRH Receptor Expression in Pharynx Carcinoma Cells
  • 09:25Conclusion

Summary

Automatic Translation

Automatic Translation

选择性标记的荧光的GnRH-I，-II和-III衍生物对于跟踪可靠的工具和量化它们的细胞摄取。这个手稿介绍实验来可视化，量化和比较在各种细胞系这些GnRH的缀合物的吸收效率。

Tags

In Vitro ImagingQuantificationDrug Targeting EfficiencyFluorescently Labeled GnRH AnaloguesHuman Cancer Cell CulturesTargeted Anti-cancer TherapyGnRH ReceptorsConfocal MicroscopyFlow CytometryGnRH FITCPreheated Treating MediaFixing SolutionFluorescent ProbeMounting Medium

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