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In vitro Imaging og Kvantificering af Drug Targeting Effektivitet af fluorescens-mærkede GnRH-analoger
JoVE Journal
Cancer Research
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JoVE Journal Cancer Research
In Vitro Imaging and Quantification of the Drug Targeting Efficiency of Fluorescently Labeled GnRH Analogues
DOI:

10:36 min

March 21, 2017

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Chapters

  • 00:05Title
  • 01:07GnRH Uptake
  • 02:39Type I GnRH Receptor Expression
  • 05:39Fluorescence Microscopy Imaging
  • 06:26FACS-assisted GnRH Quantification
  • 08:00Results: GnRH I Uptake and Type I GnRH Receptor Expression in Pharynx Carcinoma Cells
  • 09:25Conclusion

Summary

Automatic Translation

Selektivt mærket fluorescerende GnRH-I, -II og-III derivater er pålidelige værktøjer til sporing og kvantificering deres cellulære optagelse. Dette håndskrift introducerer eksperimenter for at visualisere, kvantificere og sammenligne optagelsen effektiviteten af ​​disse GnRH konjugater i forskellige cellelinier.

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