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07:46 min
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March 27, 2017
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Here, we describe a rapid and flexible protocol for the formation of 3D cell spheroids through cell aggregation. This is easily adapted to multiple cell types and is suitable for use in a variety of applications including cell migration, invasion, or anoikis assays, and for imaging and quantifying cell-matrix interactions.
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Cite this Article
Maritan, S. M., Lian, E. Y., Mulligan, L. M. An Efficient and Flexible Cell Aggregation Method for 3D Spheroid Production. J. Vis. Exp. (121), e55544, doi:10.3791/55544 (2017).
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