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Journal / Developmental Biology / Recombinase aracılı Kaset Değişimi Kullanarak…
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Developmental Biology

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JoVE Journal Developmental Biology
Recombinase aracılı Kaset Değişimi Kullanarak Fare Embriyonik Kök Hücre yapı-fonksiyon Çalışmaları
Using Ex Vivo Upright Droplet Cultures of Whole Fetal Organs to Study Developmental Processes during Mouse Organogenesis
Journal (Developmental Biology)
Using Ex Vivo Upright Droplet Cultures of Whole Fetal Organs to Study Developmental Processes during Mouse Organogenesis
Engineering Transplantation-suitable Retinal Pigment Epithelium Tissue Derived from Human Embryonic Stem Cells
Journal (Developmental Biology)
Engineering Transplantation-suitable Retinal Pigment Epithelium Tissue Derived from Human Embryonic Stem Cells
 
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Recombinase aracılı Kaset Değişimi Kullanarak Fare Embriyonik Kök Hücre yapı-fonksiyon Çalışmaları

Article DOI: 10.3791/55575 • 15:13 min
April 27th, 2017 •
1,2,3,4, 1,2, 1,2,4, 1,2,4, 1,2, 5, 6, 1,2,4, 7, 1,2,4, 1,2,3,4
1Department of Biomedical Molecular Biology, Ghent University, 2Inflammation Research Center, VIB, 3Center for Medical Genetics, Ghent University Hospital, 4Cancer Research Institute Ghent (CRIG), 5Department of Basic Medical Sciences, Faculty of Medicine and Health Sciences, Ghent University, 6Helmholtz Center for Infection Research, 7Mammalian Functional Genetics Laboratory, Division of Blood Cancers, Australian Centre for Blood Diseases, Department of Clinical Haematology, Monash University and Alfred Health Alfred Centre

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April 27th, 2017

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Proteinler genellikle farklı hücresel fonksiyonları sarf edebilirsiniz birden çok etki içerirler. Gen irtibat kutusu kapağı (KO) bu işlevsel çeşitliliği dikkate almaz. Burada, çeşitli işlevsel etki alanları veya bir proteinin varyantlarının moleküler diseksiyon sağlar KO embriyonik kök hücrelerin bir rekombinasyon aracılı kaset değişimi (RMCE) tabanlı bir yapı-işlev yaklaşım sunulmuştur.

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