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Developmental Biology

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在小鼠胚胎干细胞结构功能研究使用重组酶介导的盒式交换
 

在小鼠胚胎干细胞结构功能研究使用重组酶介导的盒式交换

Article doi: 10.3791/55575
April 27th, 2017

Summary April 27th, 2017

Please note that all translations are automatically generated.

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蛋白质常含有可发挥不同的细胞功能多个域。基因敲除(KO)不考虑这个功能的多样性。这里,我们报告在KO胚胎干细胞的重组介导的盒式交换(RMCE)系的结构 - 功能的方法,其允许各种功能结构域或蛋白质的变体的分子清扫。

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