A subscription to JoVE is required to view this content. Sign in or start your free trial.
1Flow Cytometry Core Facility, Center for Translational Research-Technical Core,Institut Pasteur, 2Unit for Lymphopoiesis, Immunology Department, INSERM U1223,University Paris Diderot, Sorbonne Paris Cité, Cellule Pasteur, Institut Pasteur, 3Stem-Cell Microenvironments in Repair/Regeneration Team,Instituto de Investigação e Inovação em Saúde (i3s), INEB – Instituto de Engenharia Biomédica, 4ICBAS – Instituto de Ciências Biomédicas Abel Salazar,Universidade do Porto, 5Stem Cells and Regenerative Medicine Team, UMRS 1166, ICAN – Institute of Cardiometabolism And Nutrition,UPMC – Université Pierre et Marie Curie – Paris 6, INSERM
Chapters
- 00:05Title
- 01:07Intestinal Cells Isolation
- 02:20Cell Staining
- 03:26Preparation of Cell Suspension from Embryonic Mouse Heart
- 05:31Cardiac Cell Staining
- 06:54Data Acquisition and Analysis
- 08:44Results: Spectral Cytometry-based Characterization of Embryonic Cardiac Cells
- 09:50Conclusion
Denne artikel beskriver spektrale cytometri, en ny tilgang i flowcytometri, der bruger figurer af emission spektre til at skelne fluorokromer. En algoritme erstatter kompensationer og kan behandle auto-fluorescens som en selvstændig parameter. Denne nye tilgang giver mulighed for en korrekt analyse af celler isoleret fra faste organer.
