Analysis of Cell Suspensions Isolated from Solid Tissues by Spectral Flow Cytometry

Sandrine Schmutz; Mariana Valente; Ana Cumano; Sophie Novault

doi:10.3791/55578

Journal

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Biology

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Spektral Sitometrisi ile Katı Dokular İzole Hücre Cezalar Analizi

Analysis of Cell Suspensions Isolated from Solid Tissues by Spectral Flow Cytometry

JoVE Journal
Biology

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JoVE Journal Biology

Analysis of Cell Suspensions Isolated from Solid Tissues by Spectral Flow Cytometry

Spektral Sitometrisi ile Katı Dokular İzole Hücre Cezalar Analizi

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12,940 Views

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11:08 min

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May 05, 2017

DOI:

10.3791/55578-v

DOI:

10.3791/55578-v

•

11:08 min

May 05, 2017

•

12937 Views

Sandrine Schmutz¹, Mariana Valente^2,3,4,5, Ana Cumano², Sophie Novault¹

¹Flow Cytometry Core Facility, Center for Translational Research-Technical Core,Institut Pasteur ²Unit for Lymphopoiesis, Immunology Department, INSERM U1223,University Paris Diderot, Sorbonne Paris Cité, Cellule Pasteur, Institut Pasteur ³Stem-Cell Microenvironments in Repair/Regeneration Team,Instituto de Investigação e Inovação em Saúde (i3s), INEB – Instituto de Engenharia Biomédica ⁴ICBAS – Instituto de Ciências Biomédicas Abel Salazar,Universidade do Porto ⁵Stem Cells and Regenerative Medicine Team, UMRS 1166, ICAN – Institute of Cardiometabolism And Nutrition,UPMC – Université Pierre et Marie Curie – Paris 6, INSERM

Summary

Bu makalede, sitometrisi spektral, fluorochromes ayırt emisyon spektrumları şekilleri kullanan akış sitometrisi yeni bir yaklaşım tarif edilmektedir. Bir algoritma, tazminat değiştirir ve bağımsız bir parametre olarak otomatik floresan tedavi edebilir. Bu yeni yaklaşım, katı organ izole edilmiş hücre uygun analizi sağlar.