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小鼠小肠的混合原代培养拟肠激素分泌的研究和活细胞成像的肠内分泌细胞的供
JoVE Journal
Biology
This content is Free Access.
JoVE Journal
Biology
Mixed Primary Cultures of Murine Small Intestine Intended for the Study of Gut Hormone Secretion and Live Cell Imaging of Enteroendocrine Cells
Please note that all translations are automatically generated.
Click here for the English version.
小鼠小肠的混合原代培养拟肠激素分泌的研究和活细胞成像的肠内分泌细胞的供
DOI:
10.3791/55687-v
•
09:16 min
•
April 20, 2017
•
Arianna Psichas*
1
,
Gwen Tolhurst*
1
,
Cheryl A. Brighton
,
Fiona M. Gribble
,
Frank Reimann
1
Metabolic Research Laboratories and MRC Metabolic Diseases Unit
,
Wellcome Trust-MRC Institute of Metabolic Science, University of Cambridge
Chapters
00:05
Title
00:54
Tissue Preparation
03:15
Removal of Cell Debris and Single Cells
04:44
Collection of Crypt Fragments
06:55
Intestinal Culture Plating
07:38
Results: Secretion and Imaging Using Primary Small Intestinal Cultures
08:36
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
这个协议描述混合鼠小肠细胞的分离和培养。这些一次肠培养使底层使用多种技术肠肽分泌信号转导途径的研究。
Tags
Enteroendocrine Cells
Gut Hormone Secretion
Live Cell Imaging
Mixed Primary Cultures
Murine Small Intestine
Intestinal Epithelium
Intestinal Contents
Muscle Layer
Epithelium
Chyme
Mucus
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