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Developmental Biology
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分离的原发性上皮细胞从附睾的全细胞贴片钳记录
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Developmental Biology
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JoVE Journal
Developmental Biology
Whole-cell Patch-clamp Recordings of Isolated Primary Epithelial Cells from the Epididymis
Please note that all translations are automatically generated.
Click here for the English version.
分离的原发性上皮细胞从附睾的全细胞贴片钳记录
DOI:
10.3791/55700-v
•
11:52 min
•
August 03, 2017
•
Bao Li Zhang
2,3
,
Da Yuan Gao
2,3
,
Xiao Xu Zhang
3
,
Shuo Shi
,
Winnie Shum
1
School of Life Science and Technology
,
ShanghaiTech University
,
2
Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences
,
Chinese Academy of Sciences
,
3
University of Chinese Academy of Sciences
,
4
Shanghai Institute for Advanced Immunochemical Studies
,
ShanghaiTech University
Chapters
00:05
Title
00:59
Preparation of Dissection Instruments and Reagents
02:10
Isolation of Epithelial Cells from Rat Cauda Epididymides
06:09
Patch-clamp Experiment
08:32
Results: Typical Whole-cell Curent in Single Epididymal Principal Cells
10:03
Conclusion
Summary
Automatic Translation
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Automatic Translation
我们提出了一种协议,结合细胞分离和全细胞膜片钳记录来测量主要解离的上皮细胞从大鼠cauda附睾的电性质。该方案允许研究原发性附睾上皮细胞的功能特性,以进一步阐明附睾的生理作用。
Tags
Whole-cell Patch-clamp
Primary Epithelial Cells
Epididymis
Reproductive Biology
Sperm Maturation
Paternal Traits
Electrical Properties
Collagenase
Cell Dissociation
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