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分离的原发性上皮细胞从附睾的全细胞贴片钳记录
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Developmental Biology
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JoVE Journal Developmental Biology
Whole-cell Patch-clamp Recordings of Isolated Primary Epithelial Cells from the Epididymis
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分离的原发性上皮细胞从附睾的全细胞贴片钳记录

DOI:
10.3791/55700-v

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11:52 min

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August 03, 2017

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, , , ,
1School of Life Science and Technology,ShanghaiTech University, 2Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences,Chinese Academy of Sciences, 3University of Chinese Academy of Sciences, 4Shanghai Institute for Advanced Immunochemical Studies,ShanghaiTech University

Chapters

  • 00:05Title
  • 00:59Preparation of Dissection Instruments and Reagents
  • 02:10Isolation of Epithelial Cells from Rat Cauda Epididymides
  • 06:09Patch-clamp Experiment
  • 08:32Results: Typical Whole-cell Curent in Single Epididymal Principal Cells
  • 10:03Conclusion

Summary

Automatic Translation

Automatic Translation

我们提出了一种协议，结合细胞分离和全细胞膜片钳记录来测量主要解离的上皮细胞从大鼠cauda附睾的电性质。该方案允许研究原发性附睾上皮细胞的功能特性，以进一步阐明附睾的生理作用。

Tags

Whole-cell Patch-clampPrimary Epithelial CellsEpididymisReproductive BiologySperm MaturationPaternal TraitsElectrical PropertiesCollagenaseCell Dissociation

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