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Developmental Biology
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将人诱导的多能干细胞分化为神经元和神经胶质的混合培养物进行神经毒性测试的方案
JoVE Journal
Developmental Biology
This content is Free Access.
JoVE Journal
Developmental Biology
Protocol for the Differentiation of Human Induced Pluripotent Stem Cells into Mixed Cultures of Neurons and Glia for Neurotoxicity Testing
Please note that all translations are automatically generated.
Click here for the English version.
将人诱导的多能干细胞分化为神经元和神经胶质的混合培养物进行神经毒性测试的方案
DOI:
10.3791/55702-v
•
09:02 min
•
June 09, 2017
•
Francesca Pistollato
,
David Canovas-Jorda
,
Dimitra Zagoura
,
Anna Price
1
Directorate F – Health, Consumers and Reference Materials
,
Joint Research Centre
Chapters
00:05
Title
01:10
Human Induced Pluripotent Stem Cell (hiPSC) Expansion
02:20
HiPSC Differentiation into Mixed Neurons and Glia: Generation of Embryoid Bodies
03:41
Generation of Neuropithelial Aggragates
04:53
Aggregate Dissociation and Neuronal Differentiation
06:36
Results: Differentiation of IMR90-hiPSCs into Mixed Cultures of Neurons and Glia
07:53
Conclusion
Summary
Automatic Translation
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Automatic Translation
人类诱导的多能干细胞(hiPSC)被认为是用于药物和化学筛选以及开发用于毒性测试的新
体外
模型(包括神经毒性)的有力工具。这里描述了将hiPSC分化为神经元和神经胶质的详细方案。
Tags
Human Induced Pluripotent Stem Cells
Neural Stem Cells
Neuronal Differentiation
Glial Differentiation
Mixed Cultures
Neurotoxicity Testing
In Vitro Model
Drug And Chemical Toxicity Screening
Signaling Pathways
HiPSC Medium
EB Medium
Colony Fragments
Matrix-coated Dishes
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