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Journal
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Biochemistry
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寡肽竞争测定磷酸化位点测定
JoVE Journal
Biochemistry
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JoVE Journal
Biochemistry
Oligopeptide Competition Assay for Phosphorylation Site Determination
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寡肽竞争测定磷酸化位点测定
DOI:
10.3791/55708-v
•
09:16 min
•
May 18, 2017
•
Min Sung Joo
,
Ja Hyun Koo
,
Sol-Bi Shin
,
Hyungshin Yim
,
Sang Geon Kim
1
College of Pharmacy and Research Institute of Pharmaceutical Sciences
,
Seoul National University
,
2
College of Pharmacy and Institute of Pharmaceutical Science and Technology
,
Hanyang University
Chapters
00:05
Title
01:05
Site-directed Mutagenesis of pGEX-Nrf2
03:45
In Vitro
Competitive 5′ Adenosine Monophosphate-activated Protein Kinase (AMPK) Assay
06:13
In Vitro
AMPK Activity Assay with a Site-specific Mutant
07:39
Results:
In Vitro
AMPK Assays with Competitive Oligopeptides or a Site-specific Mutant Protein
08:31
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
肽竞争测定广泛用于各种分子和免疫学实验。本文描述了
体外
寡肽竞争激酶测定的详细方法和相关的验证程序,其可用于发现特定的磷酸化位点。
Tags
Oligopeptide Competition Assay
Phosphorylation Site Determination
Cell Signaling
Post-translational Modifications
Site-directed Mutagenesis
PCR
DpnI Digestion
Bacterial Transformation
DNA Sequencing
Protein Expression And Purification
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