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Ex Vivo Imaging dei linfociti T CD8 residente nel tumore del polmone umano mediante microscopia confocale a fette
This content is Free Access.
1Institut Cochin, Inserm U1016-CNRS UMR8104,Université Paris Descartes, 2Department of Pathology, Paris Centre University Hospitals,Université Paris Descartes, 3INSERM U1138, Cancer and Immune Escape, Cordeliers Research Center,University Pierre and Marie Curie, 4Department of Thoracic Surgery, Paris Centre University Hospitals,University Paris Descartes
Chapters
- 00:05Title
- 01:01Agarose Embedding and Vibratome Slicing of Human Lung Tumors
- 03:51Immunostaining of Resident CD8 T Cells and Tumor Cells
- 05:07Imaging and Analyzing Resident CD8 T Cell Migration Within Human Lung Tumor Slices
- 07:03Results: Distribution and Motility Patterns of Resident CD8 T Cells in Human Lung Tumor Tissues
- 07:53Conclusion
Questo protocollo descrive un metodo per immagine residente tumore-infiltrazione CD8 T cellule identificate con gli anticorpi fluorescente accoppiati all'interno di fette di tumore del polmone umano. Questa tecnica permette in tempo reale analisi di migrazione delle cellule T CD8 usando la microscopia confocal.
