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Developmental Biology
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分化软骨细胞与外周血源性人诱导多能干细胞
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Developmental Biology
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JoVE Journal
Developmental Biology
Differentiating Chondrocytes from Peripheral Blood-derived Human Induced Pluripotent Stem Cells
Please note that all translations are automatically generated.
Click here for the English version.
分化软骨细胞与外周血源性人诱导多能干细胞
DOI:
10.3791/55722-v
•
07:51 min
•
July 18, 2017
•
Yueying Li
,
Yong Hai
,
Jiayu Chen
,
Tie Liu
1
Department of Orthopedics, Beijing Chao-Yang Hospital
,
Capital Medical University
,
2
Key Laboratory of Genomic and Precision Medicine, Beijing Institute of Genomics
,
Chinese Academy of Sciences
,
3
Clinical and Translational Research Center of Shanghai First Maternity & Infant Hospital, School of Life Sciences and Technology
,
Tongji University
Chapters
00:05
Title
00:47
Embryoid Body (EB) Formation
03:37
Cell Pellet Formation and Chondrocyte Differentiation
05:42
Results: Representative hiPSC-Chon Pellet Characterization
06:54
Conclusion
Summary
Automatic Translation
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Automatic Translation
我们提出了一种通过诱导多能干细胞(iPSCs)从人外周血(PB)产生软骨形态谱的方案,使用无积分法,其包括胚状体(EB)形成,成纤维细胞扩增和软骨形成诱导。
Tags
Chondrocytes
Peripheral Blood
Induced Pluripotent Stem Cells
IPS Cells
Cartilage Regeneration
Chondrogenic Differentiation
Serum-free
Xeno-free
Embryoid Bodies
Fibroblastic Cells
Gelatin
Basal Culture Medium
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