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Developmental Biology
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末梢血由来ヒト誘導多能性幹細胞からの軟骨細胞の分化
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Developmental Biology
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JoVE Journal
Developmental Biology
Differentiating Chondrocytes from Peripheral Blood-derived Human Induced Pluripotent Stem Cells
Please note that all translations are automatically generated.
Click here for the English version.
末梢血由来ヒト誘導多能性幹細胞からの軟骨細胞の分化
DOI:
10.3791/55722-v
•
07:51 min
•
July 18, 2017
•
Yueying Li
,
Yong Hai
,
Jiayu Chen
,
Tie Liu
1
Department of Orthopedics, Beijing Chao-Yang Hospital
,
Capital Medical University
,
2
Key Laboratory of Genomic and Precision Medicine, Beijing Institute of Genomics
,
Chinese Academy of Sciences
,
3
Clinical and Translational Research Center of Shanghai First Maternity & Infant Hospital, School of Life Sciences and Technology
,
Tongji University
Chapters
00:05
Title
00:47
Embryoid Body (EB) Formation
03:37
Cell Pellet Formation and Chondrocyte Differentiation
05:42
Results: Representative hiPSC-Chon Pellet Characterization
06:54
Conclusion
Summary
Automatic Translation
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Automatic Translation
我々は、胚様体(EB)形成、線維芽細胞増殖および軟骨形成誘導を含む無組み込み法を用いて、誘導末梢血(PB)から誘導された多能性幹細胞(iPSC)を介して軟骨系統を生成するためのプロトコールを提示する。
Tags
Chondrocytes
Peripheral Blood
Induced Pluripotent Stem Cells
IPS Cells
Cartilage Regeneration
Chondrogenic Differentiation
Serum-free
Xeno-free
Embryoid Bodies
Fibroblastic Cells
Gelatin
Basal Culture Medium
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