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Developmental Biology
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使用Sholl分析方法量化大鼠乳腺组织中的分支密度
JoVE Journal
Developmental Biology
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JoVE Journal
Developmental Biology
Quantifying Branching Density in Rat Mammary Gland Whole-mounts Using the Sholl Analysis Method
Please note that all translations are automatically generated.
Click here for the English version.
使用Sholl分析方法量化大鼠乳腺组织中的分支密度
DOI:
10.3791/55789-v
•
11:02 min
•
July 12, 2017
•
Jason P. Stanko
,
Suzanne E. Fenton
1
National Toxicology Program Laboratory, Division of the National Toxicology Program
,
National Institute of Environmental Health Sciences
Chapters
00:05
Title
00:41
Preparing the Whole-mount Mammary Gland Image
05:48
The Sholl Analysis
08:15
Measuring the Mammary Epithelial Area (MEA)
09:05
Results: Interpreting the Data
10:23
Conclusion
Summary
Automatic Translation
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Automatic Translation
通常使用描述性评估或通过测量基本物理属性来评估啮齿动物中的乳腺发育。分支密度是乳腺发育的一个指标,难以客观量化。该方案描述了乳腺分支特征定量评估的可靠方法。
Tags
Mammary Gland
Branching Density
Branching Decay
Sholl Analysis
Computer-assisted Method
Whole-mount Images
ImageJ
Glandular Epithelium
Lymph Node
Color Channels
Background Subtraction
Unsharp Mask
Noise Removal
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