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1UCLA AIDS Institute,University of California at Los Angeles (UCLA), 2Department of Microbiology, Immunology, & Molecular Genetics,University of California at Los Angeles (UCLA), 3Departments of Biomathematics and Mathematics,University of California at Los Angeles (UCLA), 4Personalized Genomic Medicine Research Center, Division of Strategic Research Groups,Korea Research Institute of Bioscience and Biotechnology, 5Department of Medicine,University of California at Los Angeles (UCLA), 6Department of Veterinary Biosciences, College of Veterinary Medicine,The Ohio State University (OSU)
Chapters
- 00:05Title
- 01:11LTR-specific Biotin-primer Extension
- 02:53Rsal and CviQI Digestion and Blunt-ending
- 04:12Straptavidin Bead Binding
- 05:31Linker Ligation
- 06:39Pre-amplification of Both Left and Right Junction DNA
- 08:15Left- and Right-junction-specific Amplifications
- 10:23Results: The IS Bidirectional Assay Improves Detection Rates and Sequence Quantification Accuracy
- 12:09Conclusion
Este manuscrito descreve o procedimento experimental e análise de software para um ensaio de site de integração bidirecional que pode simultaneamente analisar DNA de junção vetor-hospedeiro a montante e a jusante. Os produtos de PCR bidirecionais podem ser usados para qualquer plataforma de seqüência a jusante. Os dados resultantes são úteis para uma comparação quantitativa de alto rendimento de alvos de DNA integrados.
