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基于蛋白质的继承在
酿酒酵母
中的高通量筛选
JoVE Journal
Biology
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JoVE Journal
Biology
High-throughput Screening for Protein-based Inheritance in
S. cerevisiae
Please note that all translations are automatically generated.
Click here for the English version.
基于蛋白质的继承在
酿酒酵母
中的高通量筛选
DOI:
10.3791/56069-v
•
08:12 min
•
August 08, 2017
•
James S. Byers
,
Daniel F. Jarosz
2
1
Department of Developmental Biology
,
Stanford University School of Medicine
,
2
Department of Chemical and Systems Biology
,
Stanford University School of Medicine
Chapters
00:05
Title
00:33
Initial Overexpression
03:12
Identifying Cultures Affected by the Stressor
05:13
Results: Identification of ORFs that Modulate Protein-based Inheritance
06:45
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
此协议说明一种高通量方法来功能屏幕在
酿酒酵母
中的蛋白质基础继承。
Tags
High-throughput Screening
Protein-based Inheritance
S. Cerevisiae
Prion
Protein Over-expression
Phenotypic Memory
96-well Plate
384-well Plate
Liquid Handling Robot
Optical Density
Manganese Chloride
SD-URA
SCal-URA
ST-URA
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