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Journal
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Developmental Biology
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斑马鱼视网膜蛋白定位的相关超分辨和电镜观察
JoVE Journal
Developmental Biology
This content is Free Access.
JoVE Journal
Developmental Biology
Correlative Super-resolution and Electron Microscopy to Resolve Protein Localization in Zebrafish Retina
Please note that all translations are automatically generated.
Click here for the English version.
斑马鱼视网膜蛋白定位的相关超分辨和电镜观察
DOI:
10.3791/56113-v
•
12:28 min
•
November 10, 2017
•
José M. Mateos
,
Gery Barmettler
,
Jana Doehner
,
Irene Ojeda Naharros
,
Bruno Guhl
,
Stephan C.F. Neuhauss
,
Andres Kaech
,
Ruxandra Bachmann-Gagescu
3
,
Urs Ziegler
1
Center for Microscopy and Image Analysis
,
University of Zurich
,
2
Institute for Molecular Life Sciences
,
University of Zurich
,
3
Institute for Medical Genetics
,
University of Zurich
Chapters
00:05
Title
01:12
Gelatin Infiltration, Mounting, and Cryosectioning
03:47
Immunolabeling
05:26
Super-resolution Microscopy
07:25
Platinum Shadowing and Scanning Electron Microscopy
08:48
Alignment of Light and Electron Microscopy Images
10:23
Results: Tom20 Protein Expression in the Zebrafish Retina
11:29
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
该协议描述了在斑马鱼视网膜上获得亚细胞蛋白定位结果的必要步骤, 方法是将超分辨光显微镜和扫描电子显微镜图像关联起来。
Tags
Super-resolution Microscopy
Electron Microscopy
Protein Localization
Zebrafish Retina
Tokuyasu Sections
Platinum Shadowing
Gelatin Embedding
Sucrose Cryoprotection
Cryo-ultramicrotomy
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