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流动 Cytometry-based 药物筛选系统对促进细胞分化胶质母细胞瘤小分子的鉴定
JoVE Journal
Cancer Research
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JoVE Journal Cancer Research
Flow Cytometry-based Drug Screening System for the Identification of Small Molecules That Promote Cellular Differentiation of Glioblastoma Stem Cells
DOI:

10:28 min

January 10, 2018

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Chapters

  • 00:05Title
  • 01:17Generation of Lentiviral Transcriptional Reporter Cell Lines
  • 03:47Subclone Selection, Expansion, and Validation
  • 04:47Determination of GFAP:GFP Expression by Flow Cytometry
  • 05:58ELDA Self-renewal Assay to Assess Clonogenic Capacity
  • 07:01Drug Screen
  • 08:27Results: Selection and Drug Screening of Glioblastoma Stem Cell Subclones
  • 09:46Conclusion

Summary

Automatic Translation

提出了一种有效的筛选方案, 以识别小分子, 促进形分化的胶质母细胞瘤干细胞 (GSCs)。该检测是基于一个干细胞分化的记者, 即增强 GFP (eGFP) 的表达是由人胶质细胞促进剂驱动。

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