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Flöde flödescytometri-baserade Drug Screening System för identifiering av små molekyler som främjar celldifferentiering av Glioblastoma stamceller
JoVE Journal
Cancer Research
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JoVE Journal Cancer Research
Flow Cytometry-based Drug Screening System for the Identification of Small Molecules That Promote Cellular Differentiation of Glioblastoma Stem Cells
DOI:

10:28 min

January 10, 2018

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Chapters

  • 00:05Title
  • 01:17Generation of Lentiviral Transcriptional Reporter Cell Lines
  • 03:47Subclone Selection, Expansion, and Validation
  • 04:47Determination of GFAP:GFP Expression by Flow Cytometry
  • 05:58ELDA Self-renewal Assay to Assess Clonogenic Capacity
  • 07:01Drug Screen
  • 08:27Results: Selection and Drug Screening of Glioblastoma Stem Cell Subclones
  • 09:46Conclusion

Summary

Automatic Translation

En effektiv screening protokoll presenteras för identifiering av små molekyler som främjar astroglial differentiering i glioblastoma stamceller (GSCs). Analysen är baserad på stamcells differentiering reporter varvid uttrycket av den förbättrade GFP (andra) drivs av mänskliga Fredsgenomförande arrangören.

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