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Multi foton Time Lapse Imaging te visualiseren van ontwikkeling in realtime: visualisatie van migreren neurale kamcellen in Zebrafish embryo's
JoVE Journal
Developmental Biology
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JoVE Journal Developmental Biology
Multi-Photon Time Lapse Imaging to Visualize Development in Real-time: Visualization of Migrating Neural Crest Cells in Zebrafish Embryos
DOI:

10:13 min

August 09, 2017

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Chapters

  • 00:05Title
  • 00:48Embryo Collection for Time-lapse Imaging
  • 01:51Embryo Preparation
  • 02:52Preparation of 2% Low-melt Agarose and Mounting the Embryo
  • 04:34Adjusting Software Settings on the Multi-photon Microscope and Time-lapse Acquisition
  • 07:26Post-acquisition Processing
  • 08:10Results: Multi-photon Time-lapse Imaging of Migrating Neural Crest Cells in Zebrafish Embryos
  • 09:29Conclusion

Summary

Automatic Translation

Een combinatie van de geavanceerde optische technieken van laser scanning microscopie met lange golflengte fluorescentie multi foton excitatie werd uitgevoerd om vast te leggen van real-time, hoge resolutie, driedimensionale beeldvorming van neurale crest migratie in GS (sox10:EGFP) en GS (foxd3:GFP) zebrafish embryo's.

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