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Flera Photon Time Lapse Imaging att visualisera utvecklingen i realtid: visualisering av migrera Neural Crest celler hos zebrafiskar embryon
JoVE Journal
Developmental Biology
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JoVE Journal Developmental Biology
Multi-Photon Time Lapse Imaging to Visualize Development in Real-time: Visualization of Migrating Neural Crest Cells in Zebrafish Embryos
DOI:

10:13 min

August 09, 2017

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Chapters

  • 00:05Title
  • 00:48Embryo Collection for Time-lapse Imaging
  • 01:51Embryo Preparation
  • 02:52Preparation of 2% Low-melt Agarose and Mounting the Embryo
  • 04:34Adjusting Software Settings on the Multi-photon Microscope and Time-lapse Acquisition
  • 07:26Post-acquisition Processing
  • 08:10Results: Multi-photon Time-lapse Imaging of Migrating Neural Crest Cells in Zebrafish Embryos
  • 09:29Conclusion

Summary

Automatic Translation

En kombination av avancerade optiska tekniker för laserscanning mikroskopi med lång våglängd flera photon fluorescens excitation implementerades för att fånga högupplösta, tredimensionella, realtid avbildning av neural crest migration i Tg (sox10:EGFP) och Tg (foxd3:GFP) zebrafiskar embryon.

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