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Journal / Bioengineering / Efficiente generazione e la modifica di IPSCs…
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JoVE Journal Bioengineering
Efficiente generazione e la modifica di IPSCs privo di alimentatore da cellule pancreatiche umane utilizzando il sistema di CRISPR-Cas9
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Efficiente generazione e la modifica di IPSCs privo di alimentatore da cellule pancreatiche umane utilizzando il sistema di CRISPR-Cas9

Article DOI: 10.3791/56260-v • 09:16 min • November 8th, 2017
November 8th, 2017 •
1,2, 3, 1,2,4
1Department of Animal and Avian Sciences, University of Maryland, 2Animal Bioscience and Biotechnology Laboratory, ARS, USDA, 3NIH Stem Cell Unit, Bethesda, National Institutes of Health, 4RenOVAte Biosciences Inc

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Questo protocollo descrive in dettaglio la generazione di cellule staminali pluripotenti indotte privo di impronta (iPSCs) da cellule pancreatiche umane in condizioni prive di alimentatore, seguito da editing con CRISPR/Cas9 ribonucleoproteine e caratterizzazione del modificati cloni unicellulari.

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Bioingegneria problema 129 le cellule staminali pluripotenti indotte (iPSCs) riprogrammazione sendai virus CRISPR/Cas9 privo di impronta iPSCs nucleofection
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