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JoVE Journal
Bioengineering

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Effektiv Generation og redigering af Feeder-gratis IPSCs fra pancreas celler ved hjælp af CRISPR-Cas9 System
 
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Effektiv Generation og redigering af Feeder-gratis IPSCs fra pancreas celler ved hjælp af CRISPR-Cas9 System

Article DOI: 10.3791/56260
November 8th, 2017

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Summary November 8th, 2017

Please note that all translations are automatically generated.

Click here for the English version.

Denne protokol beskriver i detaljer generation af fodaftryk-fri induceret pluripotente stamceller (iPSCs) fra pancreas menneskeceller i feeder-fri betingelser, efterfulgt af redigering bruger CRISPR/Cas9 ribonucleoproteins og karakterisering af den modificerede encellede kloner.

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