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Genetics

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JoVE Journal Genetics
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Hoge resolutie fluorescentie In Situ hybridisatie in Drosophila embryo's en weefsels met behulp van Tyramide signaal versterking

Article DOI: 10.3791/56281
October 19th, 2017 •
1,2, 1, 1,2, 1,2
1The Terrence Donnelly Centre for Cellular and Biomolecular Research, University of Toronto, 2Department of Molecular Genetics, University of Toronto

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October 19th, 2017

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Het beschreven RNA in situ hybridisatie protocol staat toe de opsporing van RNA in hele Drosophila embryo's of ontleed weefsels. Met behulp van 96-wells microtiterplaat platen en tyramide signaal versterking, kunnen afschriften worden gedetecteerd op hoge resolutie, gevoeligheid en doorvoer, en tegen een relatief lage prijs.

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