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JoVE Journal
Genetics

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JoVE Journal Genetics
Alta resolución fluorescente In Situ hibridación en embriones de Drosophila y tejidos utilizando amplificación de la señal de Tyramide
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Alta resolución fluorescente In Situ hibridación en embriones de Drosophila y tejidos utilizando amplificación de la señal de Tyramide

Article DOI: 10.3791/56281
October 19th, 2017 •
1,2, 1, 1,2, 1,2
1The Terrence Donnelly Centre for Cellular and Biomolecular Research, University of Toronto, 2Department of Molecular Genetics, University of Toronto

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October 19th, 2017

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El RNA se describe en situ hibridación protocolo permite la detección de RNA en todo Drosophila embriones o tejidos disecados. Utilizando placas de microtitulación de 96 pocillos y amplificación de la señal tyramide, transcripciones pueden ser detectadas en el rendimiento, sensibilidad y alta resolución y a un costo relativamente bajo.

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