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Target Cell pre berigelse og hele genom forstærkning for enkelt celle Downstream karakterisering
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1Institute of Cell Biology, Histology and Embryology,Medical University of Graz, 2Institute of Pathology,Medical University of Graz, 3Fraunhofer Institute for Toxicology and Experimental Medicine ITEM, 4Department of Tumor Biology,University Medical Center Hamburg-Eppendorf, 5Sahlgrenska Cancer Center,University of Gothenburg
Chapters
- 00:05Title
- 01:28Preparation of Target Cells
- 02:41Charging the Wire
- 03:27Counting Cells on the Wire
- 04:03Detachment and Recovery of Target Cells
- 05:25Single-cell Sampling Using Micromanipulation
- 06:37Single-cell Sampling Using Laser Microdissection
- 07:47Results: Use of Functionalized Wire for Ex Vivo Characterization and Genomic Analysis at Single Cell Level
- 08:59Conclusion
Denne protokol er at restituere sig og forberede Molekylær genetisk karakterisering på niveauet encellede sjældne målceller fra en blanding med ikke-målarter baggrund celler. DNA kvalitet er lig med ubehandlede enkelt celler og giver mulighed for enkelt-celle ansøgning (begge screening baseret og målrettet analyse).
