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Journal
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Immunology and Infection
/
人类的过表达和纯化<em>顺</emβ-肾上腺素转移酶<em>大肠杆菌</em
JoVE Journal
Immunology and Infection
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JoVE Journal
Immunology and Infection
Overexpression and Purification of Human
Cis
-prenyltransferase in
Escherichia coli
Please note that all translations are automatically generated.
Click here for the English version.
人类的过表达和纯化<em>顺</emβ-肾上腺素转移酶<em>大肠杆菌</em
DOI:
10.3791/56430-v
•
07:35 min
•
August 03, 2017
•
Ilan Edri*
1
,
Michal Goldenberg*
1
,
Michal Lisnyansky
,
Roi Strulovich
,
Hadas Newman
3
,
Anat Loewenstein
3
,
Daniel Khananshvili
,
Moshe Giladi
4
,
Yoni Haitin
1
Sackler Faculty of Medicine
,
Tel Aviv University
,
2
Department of Physiology and Pharmacology, Sackler Faculty of Medicine
,
Tel Aviv University
,
3
Department of Ophthalmology, Tel Aviv Sourasky Medical Center, affiliated to the Sackler Faculty of Medicine
,
Tel Aviv University
,
4
Tel Aviv Sourasky Medical Center, affiliated to the Sackler Faculty of Medicine
,
Tel Aviv University
Chapters
00:05
Title
00:56
Purification of Human DHDDS
03:52
Analytical Size-exclusion Chromatography (SEC)
04:40
Enzyme Kinetics – Time-dependent Activity
06:15
Results: Purification and Analysis of Human DHDDS
07:00
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
来自
大肠杆菌的
非变性条件下用于过表达和纯化密码子优化的人
顺式 -
丙烯基转移酶的简单方案
大肠杆菌
,以及酶活性测定法。该方案可以推广用于生产适合于机械研究的数量和质量的其它
顺式
异戊烯基转移酶蛋白质。
Tags
Cis-prenyltransferase
Human DHDDS
Overexpression
Purification
Escherichia Coli
IMAC
Size Exclusion Chromatography
TEV Protease
Retinitis Pigmentosa
Article
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