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细胞 Bead-based 免疫分析平台对受激小鼠脾多重细胞因子的研究
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Multiplex Cytokine Profiling of Stimulated Mouse Splenocytes Using a Cytometric Bead-based Immunoassay Platform
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细胞 Bead-based 免疫分析平台对受激小鼠脾多重细胞因子的研究

DOI:
10.3791/56440-v

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11:00 min

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November 09, 2017

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, , , ,
1BioLegend

Chapters

  • 00:05Title
  • 00:58Reagent and Standard Preparation
  • 02:57Assay Procedure
  • 06:23Flow Cytometer Set-up
  • 09:24Results: Quantification of Cytokines Produced by Mouse Splenocytes Cultured Under Stimulating Conditions
  • 10:15Conclusion

Summary

Automatic Translation

Automatic Translation

该协议描述了多细胞因子靶点的定量, 同时在组织培养清收集的刺激小鼠脾使用多路微珠基免疫分析平台和流式仪。

Tags

Multiplex Cytokine ProfilingMouse SplenocytesCytometric Bead-based ImmunoassayFlow CytometryCytokine QuantificationImmunologyELISAAntibody-immobilized BeadsMouse Th Cytokine StandardSerial DilutionAssay BufferFilter PlatesWash Buffer

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