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替代体外测定病毒衣壳结构完整性的方法
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Alternative In Vitro Methods for the Determination of Viral Capsid Structural Integrity
DOI:

12:57 min

November 16, 2017

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Chapters

  • 00:05Title
  • 00:49Plate-based Binding Assay for Evaluating Loss of Higher Order Norovirus Capsid Structure
  • 04:44Dynamic Light Scattering (DLS) Detecting Virus Aggregation After Heat Treatment
  • 06:32DLS for Detecting Virus Aggregation in Real Time
  • 07:29Transmission Electron Microscopy (TEM) Sample Preparation
  • 09:34Results: Reduction of Human Norovirus Capsid Stability After Heating
  • 11:36Conclusion

Summary

Automatic Translation

利用病毒基因组扩增的常规检测方法由于不能区分传染性的非传染性微粒而受到限制。本文的目的是提供的其他方法, 以帮助区分传染性病毒颗粒使用适结合, 动态光散射和透射电子显微镜详细的协议。

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